Biologix Pre-made Electrophoresis buffers include agarose gel electrophoresis buffer and polyacrylamide gel electrophoresis buffer. Using biologix-electrophoresis buffers can help standardize your electrophoresis runs, as they are made from purity-tested reagents through biologix-electrophoresis separation, ensuring reproducibility through quality control.
Features:
High quality: The buffers are filtered by 0.4µm to ensure the highest quality.
Time-saving and error-avoiding: Pre-made electrophoresis buffers shorten preparation time while reducing the likelihood of buffer concentration errors.
select ion Guide
1.Nucleic acid electrophoresis
When high resolution is not necessary, both TAE and TBE buffers are suitable. For higher resolution requirements, using a lower concentration gel is advantageous for improving the resolution of large molecular weight nucleic acids (>13kb), and TAE is recommended. Conversely, higher concentration gels are beneficial for enhancing the resolution of small molecular weight nucleic acids, and TBE is preferable in this case.
2.Protein Electrophoresis
Neutral pH buffers for the Bis-Tris gel system help to improve sample integrity and gel stability, resulting in clearer, sharper bands and higher resolution for low protein abundance or downstream applications requiring high protein integrity. MOPs buffer is recommended for separating proteins of 15 - 260 kDa. As for the proteins of 3.5 - 160 kDa, MES buffer is recommended. Electrophoresis on Tris-Glycine gel system, TGS (TG+0.1% SDS) buffer is recommended.
3.Protein blotting
Transfusion buffers are used in WB experiments to transfer proteins from gels to membranes, and then to semi-quantitatively detect a protein in complex samples based on antigen-antibody specific binding.
| Cat.# | Product Name | Formula | Package |
| TAE-500 | TAE pH8.0 buffer(1x) | 40 mM Tris-acetate, 1 mM EDTA | 500mL/ Bottle, 10 Bottles/Case |
| TGS-500 | TGS buffer(1x) | 25 mM Tris, 192 mM Glycine, 0.1% SDS | 500mL/ Bottle, 10 Bottles/Case |
| TG-500 | TG buffer(1x) | 25 mM Tris, 192 mM Glycine | 500mL/ Bottle, 10 Bottles/Case |
| TG-510 | TG buffer(10x) | 0.25 M Tris, 1.92 M Glycine | 500mL/ Bottle, 10 Bottles/Case |
| WTB-510 | WTB transfer buffer(10x) | 0.25 M Tris, 1.92 M Glycine | 500mL/ Bottle, 10 Bottles/Case |
| MOPS-500 | Tris-MOPS buffer(1x) | 50 mM Tris, 50 mM MOPS, 1 mM EDTA, 0.1% SDS | 500mL/ Bottle, 10 Bottles/Case |
| MES-500 | Tris-MES buffer(1x) | 50 mM Tris, 50mM MES, 1 mM EDTA, 0.1% SDS | 500mL/ Bottle, 10 Bottles/Case |
| SDS-500 | SDS Buffe | 10% SDS | 500mL/ Bottle, 10 Bottles/Case |
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