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  • Trypsin-EDTA Solution
Trypsin-EDTA Solution
  • Brand:
    BIOLOGIX
  • Model:
    AB-DT101/AB-DT102
  • Material:
Sterile, Can be used directly in the digestion of cultured cells or the digestion of some tissues.
Characteristic

Biologix Trypsin is a type of serine hydrolase that cuts the carboxyl side of lysine and  arginine residues in polypeptide chains, hydrolyzes intercellular proteins, and disrupts  intercellular junctions, thereby disintegrating tissues or walled cells into individual cells.  The enzyme solution is sterile and can be used directly in the digestion of cultured cells  or the digestion of some tissues. When using trypsin, the concentration, temperature,  and time should be controlled to avoid over-digestion and cell damage.


Usage method: Before use, under sterile conditions, transfer the solution from B bottle  to A bottle, gently shake to mix well, and the trypsin will be obtained.


Storage conditions: The solution should be stored upon receipt at 2-8℃ .The product  is stable for 24 months, repeated freezing and thawing are needed to be avoided.


Product performance:

1.Digestion time: The digestion time of Biologix's trypsin solution  is similar to that of Brand G, but it is superior to that of Brand P in  terms of time.

2.Proportion of cell density increase: After digestion with Biologix's trypsin  solution, the growth multiple of different cell densities was similar to that of  Brand G, especially showing a significant growth advantage on TM4 cells.

Ordering Information


Cat.#Product NameDescriptionPackage
AB-DT1010.25% Trypsin-EDTA Solution(1x)

Dissolved in D-Hanks, 2.5g/L Trypsin,

 0.2g/L EDTA, phenol red free

50mL/Set, 5 Sets/Box, 

6 Boxes/Case

AB-DT1020.25% Trypsin-EDTA Solution(1x)

Dissolved in D-Hanks, 2.5g/L Trypsin, 

0.2g/L EDTA, with phenol red

50mL/Set, 5 Sets/Box, 

6 Boxes/Case



Quality control:

TestSpecificationMeasured
AppearanceRed transparent liquidRed transparent liquid
Visible ParticleNoNo
VirusesNegative(-)Negative(-)
FungusNegative(-)Negative(-)
pH7.2-7.4Qualified
Osmotic pressure(mOsm/kg)270-320Qualified
Endotoxin(EU/mL)<3<1

Digestion 

experiment(min)

Vero≤ 8≤ 5
HCT-116≤ 5≤ 3

Quality Control  

Conclusions

Refer to the 2020 edition of "Pharmacopoeia of the People's Republic of China"and 

the enterprise internal control standards  to determine that the product is qualified!


Online Service
Any request, Freely send message to our email: Inquiry@BiologixGroup.com