The product is a dry powder SB broth medium. The product specification is 250g per  bottle. SB medium is rich in a substantial amount of tryptone and yeast extract. The  major nutrients contained in tryptone and yeast extract far exceed what is required to  reach cell growth saturation. SB medium is mainly used for the extraction of plasmids  and proteins.

Instructions for use: 

1.Weigh 57.0 g of this product 

2.Dissolve it by heating in 1000 mL of distilled water  

3.Dispense it into aliquots 

4.Sterilize by autoclaving at 121℃ for 15 minutes before use 

Performance Data: 

The SB broth formulated by this product was compared with SB media prepared using raw materials from international Brand  O and domestic Brand S. All media were supplemented with the same final concentration of ampicillin and inoculated with  equal amounts of E. coli DH5α (containing the pUC19 plasmid) for overnight culture. The validation results are as follows.

Ingredients :