This product is a dry powder TB broth medium. The product specification is 250g per  bottle. TB medium is the recommended medium for protein expression in E. coli and is  capable of increasing protein yield. It is suitable for various expression methods, such  as IPTG induction, arabinose induction, and maltose induction. TB medium is also used  to resuscitate and preservation of lyophilized bacterial strains.

Instructions for use: 

1.Weigh 50.9g of this product 

2.Dissolve it by heating in 1000 mL of distilled water 

3.Add 4 mL of glycerol.  

4.Dispense it into aliquots 

5.Sterilize by autoclaving at 121℃ for 15 minutes before use. 

Principles of testing: The formulation of TB medium contains higher concentrations of  tryptone, yeast extract, and glycerol (as carbon source）

Performance Data: 

The TB broth formulated by this product was compared with TB media prepared using raw materials from international Brand O and  domestic Brand $. All media were supplemented with the same final concentration of ampicillin and inoculated with equal amounts  of E. coli DH5α (containing the pUC19 plasmid) for overnight culture. The validation results are as follows.

Ingredients :