The product is a dry powder 2×YT medium. The product specification is 250g per  bottle. For the cultivation of Escherichia coli.

Instructions for use:  

1.Suspend 31.0 g in 1 L of purified water 

2.Heat with frequent agitation and boil to completely dissolve the powder 

3.Dispense and autoclave at 121 ℃ for 20 minutes 

Principles of testing: Tryptone and yeast extract provide carbon source, nitrogen  source, vitamins and minerals; sodium chloride maintains balanced osmotic pressure.

Performance Data: 

The 2×YT medium formulated by this product was compared with the 2×YT medium prepared using raw materials from  international Brand O. Both media were supplemented with the same final concentration of ampicillin and inoculated with equal  amounts of E. coli DH5α (containing the pUC19 plasmid) for overnight culture. The validation results are as follows.

Ingredients :

Storage: Store in a dry place away from light, tighten the cap immediately after use.