Biologix Pre-made Electrophoresis buffers include agarose gel electrophoresis buffer  and polyacrylamide gel electrophoresis buffer. Using biologix-electrophoresis buffers  can help standardize your electrophoresis runs, as they are made from purity-tested  reagents through biologix-electrophoresis separation, ensuring reproducibility through  quality control. 

Features: 

High quality: The buffers are filtered by 0.4µm to ensure the highest quality. 

Time-saving and error-avoiding: Pre-made electrophoresis buffers shorten preparation  time while reducing the likelihood of buffer concentration errors. 

selection Guide

1.Nucleic acid electrophoresis 

When high resolution is not necessary, both TAE and TBE buffers are suitable. For higher resolution requirements, using a lower  concentration gel is advantageous for improving the resolution of large molecular weight nucleic acids (>13kb), and TAE is  recommended. Conversely, higher concentration gels are beneficial for enhancing the resolution of small molecular weight nucleic  acids, and TBE is preferable in this case. 

2.Protein Electrophoresis

Neutral pH buffers for the Bis-Tris gel system help to improve sample integrity and gel stability, resulting in clearer, sharper  bands and higher resolution for low protein abundance or downstream applications requiring high protein integrity. MOPs buffer  is recommended for separating proteins of 15 - 260 kDa. As for the proteins of 3.5 - 160 kDa, MES buffer is recommended.  Electrophoresis on Tris-Glycine gel system, TGS (TG+0.1% SDS) buffer is recommended. 

3.Protein blotting

Transfusion buffers are used in WB experiments to transfer proteins from gels to membranes, and then to semi-quantitatively  detect a protein in complex samples based on antigen-antibody specific binding.