This product is a dry powder type of LB broth culture medium. The product specification is 250g per bottle. It is used for general bacterial culture, especially for the preservation and culture of E.coli in molecular biology test.
Instructions for use:
1. Weigh out 25g of this product
2. Add distilled water or deionized water at 1 L
3. Stir and heat to boiling until completely dissolved
4. Aliquot into test tubes or triangular flasks
5.Sterilize by autoclaving at 121℃ for 15 minutes before use
Ingredients :
| Formula (1L) | Amount(g) |
| Tryptone | 10.0 |
| Sodium Chloride | 10.0 |
| Yeast Paste | 5.0 |
| Final pH (25℃ ) | 7.0±0.2 |
* Note: The reference of the formula ingredients is Molecular Cloning: A Laboratory Manual Vol.3, p.1513.
Storage: 10-30℃ , stable for 3 years before unsealing
Quality Control:
Quality Control Strains with Code | Index | Cultivation Conditions | Quality Control Evaluation Criteria |
Escherichia coli ATCC25922 | Growth rate | Culture at 36±1℃ for 18~24 h | PR ≥ 0.7, gray-white colonies |